Journal: Journal of Functional Biomaterials

Article Title: Single-Step Extrusion Printing of Microgrooved Annulus Fibrosus Scaffolds via Patterned Nozzles

doi: 10.3390/jfb17030140

Figure Lengend Snippet: ( a ) Representative images of collagen type I (green), decorin (orange), fibromodulin (red), and nuclei (DAPI, blue) in BM-MSCs (all donors) cultured on PCL scaffolds for 4 weeks in medium with (+TGF-β 3 ) or without (−TGF-β 3 ). The microgroove patterning was more faithfully retained in the absence of TGF-β 3 , where ECM deposition occurred preferentially within the concave microgrooves as organized columnar arrays. (Scale bars: 200 µm). ( b ) Quantitative measurement of sGAG per DNA on the scaffolds indicated higher sGAG production for the group cultured with TGF-β 3 in the medium. (Donor 1 dark blue circles, donor 2 green squares, donor 3 cyan triangles). * indicates p < 0.05 versus week 0, # indicates p < 0.05 between groups.

Article Snippet: Cells were incubated in blocking solution containing 10% goat serum in 0.1% PBS-Tween (0.1% Tween-20 in PBS) and stained with primary antibody against collagen type I (host mouse, 1:5000 in PBS-Tween), decorin (6D6, host mouse, 37 μg/mL, DSHB (Developmental Studies Hybridoma Bank), Iowa, USA) and fibromodulin (FMOD, host rabbit, 0.5 mg/mL, Thermo Fisher Scientific).

Techniques: Cell Culture

Journal: Science Progress

Article Title: Low-dose urapidil mitigates renal ischemia-reperfusion injury through matrix metalloproteinase-9 inhibition and anti-inflammatory effects

doi: 10.1177/00368504261438886

Figure Lengend Snippet: Comparison of MMP-9 IHC between the kidney samples from each group. Details of MMP-9 marking were observed with IHC. MMP-9, matrix metalloproteinase-9; IHC, immunohistochemistry; SH, sham group; CR, control group; I/R, ischemia-reperfusion injury; UP, urapidil.

Article Snippet: Following the completion of the blocking procedure, the samples were treated with primary antibodies, including eNOS (Boster Bio., catalog number: A01604-2, diluted at a ratio of 1:250), caspase 3 (Thermo Scıentıfıc, catalog number: RB-1197-P0, diluted at a ratio of 1:100), TNF-α (Proteintech, catalog number:60291-1-Ig, diluted at a ratio of 1:300), IL-1β (Bioss, catalog number:bs-6319R, diluted at a ratio of 1:150), IL-6 (ST John’s, catalog number:STJ1110424, diluted at a ratio of 1:100), and MMP-9 (Boster Bio, catalog number: PA2140-2, diluted at a ratio of 1:100).

Techniques: Comparison, Immunohistochemistry, Control